Gene expression profile at single cell level of iPSC-derived enteric neural lineages from Parkinson's Disease patients

iPSC-derived enteric neural lineages are a mixed culture of enteric neurons and glial cells. We used scRNAseq to dissect differences at single cell level between patients and control lines. Overall design: On day 70 of differentiation, we obtained single cells from iPSC-ENLs using a papain-DNAse digestion protocol and cells were submitted to scRNAseq analysis with 6000 cells targeted per sample and 50,000 reads targeted per cell