Table_1_Detecting QTL and Candidate Genes for Plant Height in Soybean via Linkage Analysis and GWAS.xlsx

Soybean is an important global crop for edible protein and oil, and plant height is a main breeding goal which is closely related to its plant shape and yield. In this research, a high-density genetic linkage map was constructed by 1996 SNP-bin markers on the basis of a recombinant inbred line population derived from Dongnong L13 × Henong 60. A total of 33 QTL related to plant height were identified, of which five were repeatedly detected in multiple environments. In addition, a 455-germplasm population with 63,306 SNP markers was used for multi-locus association analysis. A total of 62 plant height QTN were detected, of which 26 were detected repeatedly under multiple methods. Two candidate genes, Glyma.02G133000 and Glyma.05G240600, involving in plant height were predicted by pathway analysis in the regions identified by multiple environments and backgrounds, and validated by qRT-PCR. These results enriched the soybean plant height regulatory network and contributed to molecular selection-assisted breeding.

大豆作为一种全球重要的食用蛋白质和油脂作物，其植株高度作为主要的育种目标，与植株形态及产量密切相关。在本研究中，基于由东农L13与合农60杂交形成的重组自交系群体，构建了一个基于1996年SNP-bin标记的高密度遗传连锁图谱。共鉴定出与植株高度相关的33个数量性状位点（QTL），其中5个在多个环境中被重复检测。此外，利用含有63,306个SNP标记的455份种质群体进行了多座位点关联分析。共检测到62个与植株高度相关的数量性状等位基因（QTN），其中26个在多种方法下被重复检测。通过多环境与背景识别的区域，通过通路分析预测了两个与植株高度相关的候选基因，Glyma.02G133000和Glyma.05G240600，并通过qRT-PCR进行了验证。这些结果丰富了大豆植株高度调控网络，并有助于分子辅助选择育种。