Benchmarking dataset for plant single-cell RNA sequencing workflows in maize

This dataset supports a benchmarking study of plant single-cell RNA sequencing workflows in maize and is designed for evaluating single-cell RNA-seq performance in plant systems, including nuclei and protoplast-derived cells. This resource is relevant for plant single-cell transcriptomics, scRNA-seq method development, and full-length isoform analysis. This dataset supports the study published in Plant Methods: Wang, J., Zheng, S., Lu, B. et al. Integrated experimental and computational workflows for single-cell transcriptomics in plants. Plant Methods 22, 12 (2026). https://doi.org/10.1186/s13007-025-01490-6