Solubility and secondary structure of binary alternating peptides.

1 The secondary structure was estimated from the CD spectrum of the precipitate derived from a 0.2 mg/ml solution of peptide in 50 mM phosphate buffer pH 8. When the precipitate was transferred to the pH 4 buffer, the spectra were essentially the same.2 The amount of the peptide in the supernatant and precipitate fractions was determined by hydrolysis (see Materials and Methods).3 The CD spectra of samples indicated had no sufficient precipitate to be measured.4 The quantitation of this sample was prevented by an interfering compound (HPLC).Solubility and secondary structure of binary alternating peptides.