The data for "Comparing behaviours induced by natural memory retrieval and optogenetic reactivation of an engram ensemble in mice"

we compared two defensive behaviors (freezing and the syllable structure of ultrasonic vocalizations, USVs) induced by training cues (natural memory retrieval) and optogenetic engram ensemble reactivation (artificial memory retrieval) in a threat conditioning paradigm in the same mice. During natural memory recall, we observed a strong positive correlation between freezing levels and USV syllable characteristics (characterized by an unsupervised algorithm, MUPET, Mouse Ultrasonic Profile ExTraction). Furthermore, strikingly similar behavioural profiles were observed in the same mice when engram ensemble neurons were optogenetically reactivated. Although our analysis focused on two behavioral measures of threat memory (freezing and USV characteristics), these results underscore the similarities between threat memory recall triggered naturally and through optogenetic engram reactivation.