Intestinal interleukin-17 receptor signaling mediates reciprocal control of the gut microbiota and autoimmune inflammation. Mus musculus

Given the link between commensal microbiota and Th17 development, there is intense interest in understanding the role of IL-17 in regulating host defense genes expression in the gut. To Assess this we performed RNA sequencing on terminal ileum of the following mouse strains WT, Il17a-/-, Il17ra-/-, and intestinal epithelial specific IL-17RA knockout mice.To understand the role of IL-17 in regulating host defense gene expression in the intestinal epithelial cells. Towards this we cultured primary intestinal enteroids from C57BL/6 mice and stimulated with recombinant IL-17A for 24 hours. We performed RNA sequencing on recombinant IL-17A (50 ng/ml) stimulated enteroids.