File S1 - Genetic Variability of Microcystin Biosynthesis Genes in Planktothrix as Elucidated from Samples Preserved by Heat Desiccation during Three Decades

Supporting figures and tables. Table S1. Oligonucleotide primers used to amplify the target regions for qPCR from heat-desiccated DNA samples. Table S2. Influence of heat-desiccation of Planktothrix cells on qPCR quantification. Table S3. Average ± SE biovolume per year of the total Planktothrix population, and biovolume and proportions of mcy genotypes in Lake Zürich between 1977 and 2008. Figure S1. DNA isolated from heat-desiccated vs. frozen phytoplankton samples obtained from Lake Zürich. Figure S2. Ct values (mean ± SE) as determined by qPCR using four different gene loci of Planktothrix (16S rDNA, Dhb, Mdha and Hty genotype) from heat-desiccated DNA and treated heat-desiccated DNA. (DOCX)