Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or b
RNA-seq analysis was performed in BPDCN patient-derived cell line CAL-1 (WT and METTL3 KO) to analyze gene expression changes after loss of METTL3 concurrent with GSK595 treatment Overall design: METT
To verify whether the CcpA gene was successfully deleted, we designed the primer for PCR. The primer was designed across the upstream and downstream sequences of the CcpA gene, with a size of about 18
