Determination of amino acid sequence of a common dentex (Dentex dentex) egg yolk vitellogenin B derived protein (17.69 kDa) using liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

The 17.62 kDa vitellogenin B derived yolk protein was purified by a specific cation exchange chromatography, subjected to a liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and finally identified using short MS/MS protein signatures similar to vitellogenin of a near species from the same family, red seabream, Pagrus major, (Temminck and Schlegel, 1843).