3-phenacylideneoxindoles as a new class of antifungal compounds against Paracoccidioides spp. Supplementary material

Aims: Considering the need to identify new compounds with antifungal action, the activity of five
3-phenacylideneoxindoles compounds was evaluated. Materials & methods: The compounds were
synthesized, and their antifungal activity was elucidated through minimum inhibitory concentration
tests and interaction assay with other antifungals. Potential targets of compounds were predicted in
silico. Results: 3-phenacylideneoxindoles compounds inhibited fungal growth with minimum inhibitory
concentration and minimum fungicidal concentration ranging from 3.05 to 12.26 μM. The compounds
demonstrated high selectivity index and presented a synergistic effect with itraconazole. In silico
prediction revealed the pentafunctional AROM polypeptide, enolase, superoxide dismutase, catalase
and kinases as proteins targets of the compound 4a. Conclusion: The results demonstrate that 3-
phenacylideneoxindoles is a potential new class of antifungal compounds for paracoccidioidomycosis
treatment.