Effects of RELA and RELB in spheroid and adherent ovarian cancer

Purpose: To understand the role of NF-kB signaling in tumor-initiating cell (TIC) and non-TIC populations in ovarian cancer. Methods: OV90 cells with inducible shRNA against RelA and RelB, and a non-targeting shRNA control were induced by doxycycline treatment and were grown in adherent (non-TIC) or spheroid (TIC) conditions. Total RNA was extracted from each condtion and RNAseq was performed and analysed for differential gene expression between the conditions. Overall design: Comparison of OV90 cells grown in adherent or spheroid conditions, and the effect of silencing RelA or RelB in each condition.