Identification of CDS in the nucleotide sequence of pECTm80.

AGenBank accession numbers provided represent the results of BLAST searches (NCBI and IS BLAST server) showing the highest identity to the query sequence. The accession number for pE001 is given when there is greater than one BLAST hit at 100% ID to pECTM80. BPlasmids showing 100% ID to pECTM80 are represented as follows: 1, pE001 (JF776874.1); 2, R485 (HE577112.1); 3, pMAS2027 (FJ666132.1); 4, pOLA52 (EU370913.1); 5, pOU1114 (DQ115387.2); 6, pSE34 (EU219533.1); and 7, pMccC7-H22 (EF536825.1). Boundaries of mobile elements in the nucleotide sequence of pECTm80 are as follows: CISEc37, 7355–9182; DTn3-like, 12671–18314; FIS1R, 17240–18007. ETn3-like ΔtnpA gene showed 64% to 76% identity to transposase genes of the Tn3 family of transposons (subgroups Tn501 and Tn3) and the IS elements ISSba14, and ISSod9 of the Tn3 family.