Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes

Chromosome movement speeds during anaphase are regulated by depolymerization of microtubules. Several models describe chromosome movement during cell division but none of them consider post-translational modifications of tubulin, even though such modifications help specify microtubules for unique cellular activities. Among these modifications, acetylation of Lysine 40 is one of the common post-translational modifications. Acetylation of microtubules greatly improves their stability, especially when subjected to cooling or drug treatment. Since kinetochore microtubules are acetylated in a variety of eukaryote cells, we wondered whether deacetylation of kinetochore microtubules was necessary in order for microtubules to be able to depolymerize during anaphase. HDAC6 (Histone Deacetylase 6) deacetylates acetylated tubulin. To study whether tubulin must be deacetylated during anaphase, we added to living cells two different HDAC6 inhibitors (Tubacin and Trichostatin A), separately, as chrom..., , , # Blocking microtubules deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes

The data package contains seven datasets:

1. Fig_2_.csv
2. Figs_4.csv
3. Fig_5.csv
4. Backward_movement.csv
5. Fig_7.csv
6. Table_1_control_cells.csv
7. Table_1_treated_cells.csv

**(1) Fig_2_.csv**

This dataset contains 149N values with columns named \"Distance (microm)\" , \"Acetylated_alpha_tubulin\", and \"Tyrosinated_alpha_tubulin\". These three columns are the data before normalization of the Y axis (gray values of acetylated and tyrosinated tubulin) between 0 and 1. The next two columns, named \"Normalized_Acetylated_alpha_tubulin\" and \"Normalized_Tyrosinated_alpha_tubulin\", are the data after Y-axis normalization.

Variable names with descriptions:

* Distance (microm): shows the distance from the kinetochore of a microtubule to the pole.
* Acetylated_alpha_tubulin: shows the gray values (staining intensity) of acetylated tubulin of a fix line from the kinetochore to the pole.
*...,