HDAC1 binding regions in mouse embryonic limbs

Purpose: The response to Hedgehog signaling in the limb is driven by GLI bound enhancers and the majority of Hh targets in the developing limb bud are regulated solely by the activity of GLI-repressor. Currently we do not have a comprehensive understanding of how GLI bound enhancers respond Hedgehog signaling. The goal of this study is to identify how GLI bound enhancers are regulated by Hedgehog signaling and specifically by GLI-repressor. Methods: ChIP-seq was performed in Embryonic day 11.5 mouse forelimb and hidlimbs lfrom wildtype Swiss Webster mice. Results: We identified 15,347 HDAC1 binding sites in mouse E11.5 limb buds Overall design: ChIP-sequencing E11.5 forelimb and hindlimbs from wildtype Swiss Webster mice. Four biological replicates and three input controls were submitted.