Plant-mediated interactions between Tetranychus evansi and T. urticae spider mites - suppression test 4.

We infested all leaflets of the 3rd leaf of 29-days
old tomato plants (Solanum lycopersicum
var Castlemart) with spider mites in three treatments: (1) 20 two to four days
old adult Tetranychus evansi Viçosa-1
females, (2) 10 two to four days old adult Tetranychus
urticae Santpoort-2 females, or (3) no infestation. These treatments lasted
two days, after which we removed mites, eggs and web, and cut up to 10 leaf
discs per plant from the previously infested leaflets. Then, we placed two to
four days old adult Tetranychus evansi
Viçosa-1 females individually on each leaf disc, and counted the number of eggs
of each leaf disc after four days. These experiments were carried out in a climate
room (25°C, 16 : 8 h light : dark, 60% RH).