Phage predation in a pond microcosm leads to the selection of wbeL* phage resistant mutants.

aIndependent cultures of wild type V. cholerae O1 were used to inoculate 103 CFU per microcosm containing pond water and chitin as a sole nutrient source.bICP1 was simultaneously added at an MOI = 0.01 where indicated.cdEight isolates from each microcosm were tested for resistance to ICP1. If no alteration to sensitivity was observed, the isolates were categorized as WT. If resistance was observed than the poly-A tracts in wbeL and manA were sequenced. If resistance was observed and a frameshift in neither wbeL nor manA was observed, than the isolates were categorized as other.