Nr2f6 electroporation in tibialis anterior promotes muscle weakness

Mouse tibialis anterior muscles were electroporated with Nr2f6-myc plasmid or pCMV6 empty vector in the contralateral leg. After 9 days, muscles were collected, and the RNA extracted and processed for microarray analysis. Tibialis anterior muscle (TA) of male adult C57Bl6/J mice were electroporated with 40 ug of pCMV6-Entry empty vector as control in the left limb (L samples) and pCVM6-Nr2f2-myc-flag plasmid in the contralateral limb (R samples. After 10 days, the animals were euthanized and total RNA extracted in TRIzol and collumn purified (RNeasy Mini Kit, Qiagen).