TABLE 6 in Unravelling cryptic species diversity in eastern Nearctic paper wasps, Polistes (Fuscopolistes), using male genitalia, morphometrics and DNA barcoding, with descriptions of two new species (Hymenoptera: Vespidae)

TABLE 6. DNA barcoding: maximum intraspecific distances, distance to nearest neighbour and the 'barcoding gap' in eastern Nearctic Fuscopolistes. The upper data block deals with species in their current definition, the lower data block with major genetic clusters within currently recognized species. Taxa are sorted by decreasing Δ values (column 6). Explanations: n —number of distinct haplotypes included in the analysis; Max IntraD—maximum intraspecific distance (K2P, in %); Distance to NN—distance to nearest neighbour (K2P, in %); Barcode Gap Δ = distance to nearest neighbour minus maximum intraspecific distance (positive values indicate presence of a barcoding gap, negative values the absence thereof); Nearest Neighbour columns identify haplotypes by their GenBank numbers (all sequences are full length (658 bp) except EU649571 which is 656 bp long).
SpeciesnMax IntraDNearest SpeciesDistance to Barcode Gap Nearest NN Δ NeighbourNearest Neighbour ofdorsalis102.48carolina (A)5.86 3.38 EU649532EU649552hirsuticornis60.92fuscatus (A)3.77 2.85 JN276784EU649650parametricus50.30fuscatus (A)2.16 1.86 JN276782EU649659rubiginosus90.77fuscatus (A)2.32 1.55 EU649597EU649643bellicosus191.70carolina (A)0.46 -1.24 JN276785EU649513metricus332.65carolina (TX)0.92 -1.73 JN276801EU649621fuscatus293.62carolina (TX)1.85 -1.77 JN276843EU649601carolina184.58bellicosus0.46 -4.12 EU649513JN276785Clusterdorsalis A30.61dorsalis B1.69 1.08 JN276812EU649538fuscatus C60.92fuscatus A1.86 0.94 EU649571EU649588dorsalis B70.92dorsalis A1.69 0.77 EU649538JN276812carolina TX40.61metricus A0.92 0.31 EU649621JN276801carolina X70.92carolina TX0.92 0.001 JN276801HQ578141metricus A190.92carolina TX0.92 -0.001 JN276801EU649621fuscatus B91.08fuscatus A1.07 -0.003 JN276784HQ578143fuscatus A141.23fuscatus B1.07 -0.16 HQ578143JN276784carolina A70.92bellicosus0.46 -0.46 EU649513JN276785metricus B141.70carolina TX1.23 -0.47 JN276843EU649628carolina X/TX111.54metricus A0.92 -0.62 EU649621JN276801fuscatus A/B232.49carolina TX1.85 -0.64 JN276843EU649601