Oolong Tea Project Dataset for "Postprandial metabolic responses to high-fat feeding in healthy adults following ingestion of oolong tea-derived polymerized polyphenols: a randomized, double-blinded, placebo-controlled crossover study"
收藏Mendeley Data2024-03-27 更新2024-06-27 收录
下载链接:
https://researchdata.bath.ac.uk/id/eprint/1273
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资源简介:
10 mL blood samples collected from a canula inserted into the antecubital vein were drawn into a syringe, and immediately dispensed into untreated serum tubes with silicate clotting activator, and tripotassium ethylenediaminetetraacetic acid (K3 EDTA) treated tubes (both Sarstedt, Nümbrecht, Germany) for serum and plasma separation respectively. Before centrifugation, blood samples for serum separation were allowed to clot at room temperature for 20 minutes. Samples were centrifuged at 1300 g for 15 minutes at 4°C, then supernatant was immediately aliquoted, frozen on dry ice, and stored at -80°C for later analysis. Serum TG, and plasma TG, NEFA, glycerol, glucose, HDL-c, LDL-c, and ApoA-I, A-II, B, C-II, C-III, and E, were measured with commercially available spectrophotometric assays (Daytona Rx, Randox, Crumlin, UK) as per the manufacturer’s instructions. Commercially available enzyme linked immunosorbent assay (ELISA) was used to determine concentrations of plasma insulin (Mercodia, Uppsala, Sweden) and C-peptide (MilliporeSigma, Massachusetts, USA).
创建时间:
2023-06-28



