Oligonucleotide primer sequences used in this study with restriction endonuclease sites underlined.

Primers 1 and 2 were used to amplify HIV-1 env gene which was used to construct transfer vector pJSC1175-env from pcDNA3.1-ENV. Primers 3 and 4 were used for the identification of two recombinant vaccinia viruses. Primers 5 and 6 were used to amplify HIV-1 env gene which was used to construct the DNA vaccine pDRVISV1.0-env from pcDNA3.1-ENV.