Context-dependent effects of Acf1 assessed in high throughput
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE106759
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资源简介：
We applied a multiplexed genomic tethering assay (Brueckner, Epigenetics Chromatin, 2016; PMID:27777628) to elucidate the function of ACF1, the large subunit of the ACF nucleosome sliding complex in drosophila Kc167 cells. Targeting a Gal4DBD-ACF1 fusion to several hundred integrated reporters revealed a context-dependent repressive effect which is modulated by gene activity and chromatin state. TRIP assay in drosophila Kc167 cells combined with targeted recruitment of Gal4-Acf1 to integrated 5xGa4UAS-pMT-GFP reporters. Two technical replicate experiments of TRIP pool transfected with Gal4-Acf1, Gal4, Acf1 or Gal4-HP1 performed by day 2 after transient transfection.
创建时间：
2019-05-15
