GNPS - Effects of Dapensutrile and Glycine on mice QiitaID 15108 and 14472

Ctns -/- mice is a mouse model for cystinosis, a recessive genetic disorder in human, characterized by Fanconi syndrome and chronic kidney disease. We aim to characterize the microbiome and metabolomic phenotype from gut (fecal), urine and serum on Ctns -/- mice versus wild type controls (c57BL/6J). Dapansutrile (Qiita ID 15108) and Glycine supplementation were investigated (Qiita ID 14472). Untargeted LC-MS/MS acquisition was performed on a Vanquish UHPLC system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 50 x 2.1 mm (Phenomenex, Torrance, CA).