Thomas A Planchon, Liang Gao, Daniel E Milkie, Michael W Davidson, James A Galbraith, Catherine G Galbraith, Eric Betzig (2011) CIL:36146, Sus scrofa domestica, epithelial cell. CIL. Dataset

Views parallel and perpendicular to the mitotic plane of chromosome dynamics in a dividing LLC-PK1 cell, with a plane (left) cutting through one daughter cell during anaphase to show an interior view of the opposite chromosomes (right). Images were collected with a Bessel Beam Microscope using two photon excitation. This form of microscopy provides extraordinary 4D resolution of the separation of chromosomes during division. This video corresponds to Figure 5 and Supplementary Video 7 in Nat Methods. 2011 May;8(5):417-23. Epub 2011 Mar 4. The video is a deconvolved, maximum projection, volume rendering of slices that were collected in a 33 x 33 x 30 um3 image volume with 116 x 116 x 150 nm3 voxel sizes. The video has variable frame rates: frames 1-15 every 21 sec, frames 16-115 every 1.05 sec, frames 116-170 every 2 sec, frames 171-201 every 21 sec.

展示有丝分裂细胞中染色体动态的平行和垂直于有丝分裂平面的视角，其中一平面（左侧）在后期穿过一个子细胞，以展示对侧染色体的内部视图（右侧）。图像采用贝塞尔光束显微镜，通过双光子激发进行采集。该显微镜技术提供了对有丝分裂过程中染色体分离的非凡四维分辨率。该视频对应于《Nat Methods》2011年5月第8卷第5期第417-423页的图5和补充视频7。视频是对所采集的、以33 x 33 x 30微米立方体为体积、116 x 116 x 150纳米立方体为体素大小的切片进行去卷积、最大投影和体积渲染的结果。视频具有可变的帧率：第1-15帧每21秒一帧，第16-115帧每1.05秒一帧，第116-170帧每2秒一帧，第171-201帧每21秒一帧。