The ColR/S two-component system is a conserved determinant of host association across Pseudomonas species

Members of the bacterial genus Pseudomonas form associations with diverse hosts. Comparative transcriptomics revealed that the ColR regulon has diverged between P. aeruginosa and P. fluorescens and deleting components of the ColR regulon revealed strain-specific, but not host-specific, requirements for ColR-dependent genes. Overall design: P. fluorescens WCS365 and P. aeruginosa PAO1 wildtype and delta_colR mutants were grown in the rhizosphere or M9 minimal media. Comparative transcriptomics was performed to determine genotype and condition dependent tene expression.