Genome sequencing of European flax varieties using ONT and Illumina platforms, and BIONANO maps data

Long-read ONT sequencing data for four European flax cultivars (Ideo and Bolchoi (fiber), Marquise and Attila (oilseed)) were used to perform initial assemblies, which were polished using filtered ONT and high-quality Illumina sequence data. The polished assemblies were assembled into a hybrid scaffold using Bionano Map data and finally anchored in T2T pseudomolecule assemblies. The four assemblies were then annotated using ab initio annotation and transcriptomic evidence.