Trans-Seq for mammalian retinotectal circuit mapping

Anterograde tracing with WGA-mCherry was combined with single-cell RNA sequencing to produce bioinformatic circuit maps from retinal ganglion cell subtypes into the superior colliculus. Intraocular injections of WGA-mCherry tracer were performed to transsynaptically label postsynaptic retinorecipient neurons in the superior colliculus. The superior colliculus was processed to obtain single-cell suspensions and mCherry+ connected cells were recovered by FACS. mWmC-positive SC neurons connected to all RGCs, alpha-RGCs (labelled through the reporter Kcng4-Cre), and ooDSGCs (labelled through Cart-Cre) were isolated and profiled in this study.