Comet x 19058M

Filtered GBS data from a bi-parental mapping population between the hop powdery mildew resistant cultivar 'Comet' and susceptible USDA male line "64035M". Illumina based sequencing on a Nextseq 2000 using 150-bp sequence length was used to generate 1.3 x 10^6 SNP markers. All offspring were screened in the greenhouse for susceptibility levels to powdery mildew. Genome-wide analysis using MLM as well as QTL analyses identified one 6-Mb region on Chromosome 6 (position 308 - 314 Mb) with significant association to powdery mildew resistance. This region contains multiple clusters of resistance genes, stress response genes and other disease resistance related genes that may provide the polygenic resistance to powdery mildew.本数据集为抗啤酒花白粉病品种‘Comet’与感病USDA雄性品系‘64035M’构建的双亲作图群体的过滤后基因分型测序（Genotyping-by-Sequencing，GBS）数据。研究采用Illumina Nextseq 2000测序平台，以150bp读长进行测序，共获得1.3×10^6个单核苷酸多态性（Single Nucleotide Polymorphism，SNP）标记。所有后代群体均在温室中完成白粉病感病水平的表型鉴定。通过混合线性模型（Mixed Linear Model，MLM）开展全基因组关联分析，并结合数量性状基因座（Quantitative Trait Locus，QTL）定位分析，在第6号染色体308~314 Mb区间内鉴定到一个6 Mb的显著关联区域，该区域与白粉病抗性呈显著相关。该区域包含多组抗病基因簇、胁迫响应基因及其他病害抗性相关基因，可赋予啤酒花对白粉病的多基因抗性。