Super and typical enhancer switching in early mammalian development involves eRNA mediated SWI/SNF recruitment

Our data shows that the AT-hook domain of Brg1 is required for it's recruitment by an RNA dependent mechanism in distinct developmental stages. To further substantiate that the AT-hook Brg1 binds to the RNA, we purified the wild type and mutant AT-hook peptides and performed gel shift assay. Here, the gel images pertinent to the purification of the wild type and mutant AT-hook peptides and electrophoretic mobility shift assay (EMSA). The details of the experimental conditions for purification and EMSA are described in STAR Methods.本研究的实验数据表明，Brg1的AT-hook结构域（AT-hook domain）是其在不同发育阶段通过RNA依赖机制被招募的必需元件。为进一步验证Brg1的AT-hook结构域可与RNA结合，我们纯化了野生型与突变型AT-hook肽段，并开展了凝胶迁移实验。本数据集包含与野生型、突变型AT-hook肽段纯化以及电泳迁移率变动分析（electrophoretic mobility shift assay，EMSA）相关的凝胶成像结果。肽段纯化与EMSA实验的具体操作条件详见STAR Methods。