Tissue-specificity of IR knockdown.

Different tissues of both transgenic lines, Tet14 and Tet29, and WT were analysed for expression of IR by Western blot after treatment with doxycycline (2 mg/mL for 4 days). Quantification of the protein band intensities was carried out by the program TINA 2.08e and percentages of reduction of expression were calculated (WT, 100%). WAT, white adipose tissue; BAT, brown adipose tissue.