CLINICAL AND HISTOLOGICAL ANALYSIS OF THE EFFECT OF OZONE OIL AND LASER PHOTOBIOMODULATION ON THE TISSUE REPAIR OF TRAUMATIC ULCER ON THE RAT ORAL MUCOSA

Tissue repair is a complex event that begins at the time of injury, resulting in an attempt to restore fisiological balance. In an attempt to find alternatives that accelerate healing and consider these promising therapies for modulating this process, as well as to address the identified gap in the current literature regarding the combined use of LPBM with ozonized oil during tissue repair, no studies have addressed the combination of these agents. Thus, the aim of this study was to evaluate the effect of ozonized oil, with or without LPBM, on the healing of traumatic ulcers on the dorsal surface of the rat tongue. A total of 60 male Wistar rats underwent tongue ulceration on the dorsal surface using a six-mm diameter punch. Animals were divided into four experimental groups: control, LPBM, Ozonized oil, and LPBM + Ozonized oil. Euthanasia was performed on days three, seven, and 14 after surgery. Clinical analysis of the wound size and histomorphological evaluation using hematoxylin and eosin and Sirius red staining were conducted to identify edema, inflammatory infiltrates, fibroblastic cellularity, reepithelialization, and collagen characterization. Descriptive and exploratory analyses were performed and inferential statistics were calculated using Fisher's exact tests. Analyses were performed using R, with a significance level of 5%. LPBM and Ozonized oil positively modulated the repair phases clinically (p<0.05) and histologically, showing reepithelialization, fibroblastic population, and thick collagen fiber deposition from the 3rd day of the experiment. LPBM + Ozonized oil exhibited a significant difference from control in the clinical parameters (p<0.05), but did not demonstrate superiority in histomorphological terms of tissue repair. Isolated use of LPBM and Ozonized oil improved tissue repair in rat tongues.