CLINICAL AND HISTOLOGICAL ANALYSIS OF THE EFFECT OF OZONE OIL AND LASER PHOTOBIOMODULATION ON THE TISSUE REPAIR OF TRAUMATIC ULCER ON THE RAT ORAL MUCOSA

Tissue repair is a complex event that begins at the time of injury, resulting in an attempt to restore fisiological balance. In an attempt to find alternatives that accelerate healing and consider these promising therapies for modulating this process, as well as to address the identified gap in the current literature regarding the combined use of LPBM with ozonized oil during tissue repair, no studies have addressed the combination of these agents. Thus, the aim of this study was to evaluate the effect of ozonized oil, with or without LPBM, on the healing of traumatic ulcers on the dorsal surface of the rat tongue. A total of 60 male Wistar rats underwent tongue ulceration on the dorsal surface using a six-mm diameter punch. Animals were divided into four experimental groups: control, LPBM, Ozonized oil, and LPBM + Ozonized oil. Euthanasia was performed on days three, seven, and 14 after surgery. Clinical analysis of the wound size and histomorphological evaluation using hematoxylin and eosin and Sirius red staining were conducted to identify edema, inflammatory infiltrates, fibroblastic cellularity, reepithelialization, and collagen characterization. Descriptive and exploratory analyses were performed and inferential statistics were calculated using Fisher's exact tests. Analyses were performed using R, with a significance level of 5%. LPBM and Ozonized oil positively modulated the repair phases clinically (p&lt;0.05) and histologically, showing reepithelialization, fibroblastic population, and thick collagen fiber deposition from the 3rd day of the experiment. LPBM + Ozonized oil exhibited a significant difference from control in the clinical parameters (p&lt;0.05), but did not demonstrate superiority in histomorphological terms of tissue repair. Isolated use of LPBM and Ozonized oil improved tissue repair in rat tongues.组织修复是一类始于损伤即刻的复杂生物学过程，其核心目标是恢复机体的生理稳态。为探寻加速创面愈合的替代方案，将此类疗法视作调控组织修复进程的潜在手段，同时针对当前学界关于组织修复阶段联合应用低功率光生物调节疗法（LPBM）与臭氧化油的研究空白，目前尚无相关研究探讨这两种制剂的联合使用方案。为此，本研究旨在评估单独或联合LPBM使用臭氧化油，对大鼠舌背部创伤性溃疡愈合的影响。本研究共纳入60只雄性Wistar大鼠，采用直径6mm的打孔器在其舌背部制造创伤性溃疡模型，将大鼠随机分为4个实验组：对照组、LPBM组、臭氧化油组以及LPBM联合臭氧化油组。分别于术后第3、7、14天对大鼠实施安乐死。通过测量创面面积开展临床分析，并采用苏木精-伊红（Hematoxylin and Eosin）染色和天狼星红（Sirius red）染色进行组织形态学评估，以观察组织水肿、炎性浸润、成纤维细胞增殖、上皮再生及胶原特征。研究开展了描述性与探索性分析，并采用Fisher精确检验进行推断统计分析，所有分析均通过R软件完成，检验水准设定为5%。LPBM与臭氧化油均可在临床与组织学层面正向调控组织修复进程（p<0.05），实验第3天即可观察到上皮再生、成纤维细胞聚集及粗大胶原纤维沉积。LPBM联合臭氧化油组在临床指标上与对照组相比存在显著差异（p<0.05），但在组织修复的组织形态学层面未表现出显著优势。单独使用LPBM或臭氧化油均可改善大鼠舌部组织的修复效果。