Experimental results describing Copepod fed phytoplankton mixtures that were analyzed at San Francisco State University in 2013

<p>An approach was developed to recover prey DNA from gut contents of nauplii of a&nbsp;predatory copepod by blocking the detection of predator, <em>Tortanus dextrilobatus,</em>&nbsp;with ligase and a blocking&nbsp;oligonucleotide and amplifying prey DNA with universal primers. Adding 25 U of&nbsp;ligase improved predator clamping, while 100 and 200 U reduced efficiency. Field collected&nbsp;nauplii consumed a broad range of prey.This dataset measures the performance of the blocking oligonucleotide&nbsp;with increasing amounts of ligase and at two ratios of prey to predator DNA concentration. Data are also shown for wild nauplii at 25 and 100 U of ligase.</p>

<p>This dataset is published in Figure 2, Craig et al (2014).</p>

<p><strong>Related Reference:</strong></p>

<p>* Craig, Carrie, Wim J. Kimmerer, and C. Sarah Cohen. 2014. A DNA-based method for investigating feeding by copepod nauplii. Journal of Plankton Research 36 (1): 271-275</p>

<p>Vogt, R.A., T.R. Ignoffo, L.J. Sullivan, J. Herndon, J.H. Stillman, and W. Kimmerer. 2013. Feeding capabilities and limitations in the nauplii of two pelagic estuarine copepods, <em>Pseudodiaptomus marinus</em> and <em>Oithona davisae</em>.&nbsp; Limnology and Oceanography 58: 2145-2157.</p>