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Mass spectrometry analysis of unconventional kinetoplastid kinetochore proteins KKT1, KKT2, and KKT6

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NIAID Data Ecosystem2026-03-13 收录
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The kinetochore is the macromolecular protein machine that drives chromosome segregation in eukaryotes. Here we performed chemical crosslinking mass spectrometry of the Trypanosoma brucei KKT2 DPB/KKT1C complex and KKT1/KKT6 complex. We also performed immunoprecipitation and mass spectrometry of KKT1 fragments (KKT1N and KKT1C) that were ectopically expressed and purified from trypanosomes.

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2022-06-10
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