Pharmacokinetic measurements of BRP levels following BRP administration in mice

Raw LC-MS data (raw.d files) generated on an Agilent 6470 Triple Quadrupole LC-MS instrument to provide evidence for the pharmacokinetic measurements of BRP peptide following administration in C57BL/6J male mice. Plasma and brains were collected 0, 1,10, 30, or 60 minutes after injection of BRP (10 mg/kg I.P.). By LC-QQQ, we successfully detected endogenous peaks with transitions corresponding to the parent ions (+2 and +3 charge) in mouse plasma and brain co-eluting at 14-15 minutes with the synthesized standard. Data also show the detection of two cleaved fragments from BRP, THRIL and LFNLC, in the plasma.本数据集包含由安捷伦（Agilent）6470三重四极杆液相色谱-质谱联用仪采集的原始液相色谱-质谱联用（LC-MS）数据（扩展名为raw.d的文件），旨在为C57BL/6J雄性小鼠腹腔注射BRP肽后的药代动力学检测提供实验支撑。本研究以10 mg/kg的剂量对小鼠腹腔注射BRP，分别于给药后0、1、10、30及60分钟采集小鼠血浆与脑组织样本。通过液相色谱-三重四极杆质谱（LC-QQQ）分析，本研究成功在小鼠血浆与脑组织中检测到与合成标准品在14-15分钟处共流出、且离子跃迁对应带+2和+3电荷母离子的内源性峰。此外，数据显示在小鼠血浆中还检测到BRP的两个裂解片段：THRIL与LFNLC。