MIC values (μg/ml) of selected antibiotics against the five Scottish VREfm isolates.

Susceptibility to a given antibiotic is indicated in bold. ND: MIC value not determined. Resistant genes identified which correspond to strict or perfect matches against antibiotic resistance genes of the comprehensive antibiotic resistance database (CARD) are shown. Additionally, some loose matches (underlined) to CARD reference genes, which either had > 99% identity with reference CARD gene (tetL), or had zero e-value (rpoB2), or were the sole genes that could explain differences in antimicrobial susceptibility among the five isolates (ant(9)-Ia, marA and mtrR), are shown. The variant of a full-length penicillin-binding protein 5 gene conferring resistance to penicillins (pbp5-R), for which no reference gene in CARD exists, was found in all genomes. Superscripted numbers at top right of resistant genes (far-right column) correspond to the sequenced strains (last digit of isolate ID) carrying these genes.