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Single cell changes in DBZ or DMSO treated cells derived from Apc-mutant organoid cultures

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118055
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We made intestinal organoid cultures from Villin-CreERT2;Apcflox/flox mice. Organoids were subjected to 4-OH-Tam treatment, whereafter Apc mutant organoids were selected in R-Spondin free medium. DBZ or DMSO treatment was started on day 3. On day 8, organoids were dissociated to obtain single cells and analyzed using the Chromium Single-cell 3'RNA-sequencing system We analyzed in total 2 samples (1 DBZ and 1 DMSO), both samples contained 3000 cells
创建时间:
2019-03-08
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