The identification of starvation-responsive genes in the liver of WT and ob/ob mice.

Adaptation to starvation is a multi-molecular and temporally ordered metabolic process to optimize fuel usage, that could be impaired in obesity. However, how the liver adapts to starvation by temporally coordinating various molecules, and how the process is disrupted in obesity have not been elucidated. Here, we measured time course multi-omic data in the liver of wild-type (WT) and leptin-deficient obese (ob/ob) mice during starvation and identified the starvation-responsive genes. Ten-week-old male C57BL/6 wild-type and ob/ob mice were starved for 0, 2, 4, 6, 8, 12, 16, and 24 hours, sacrificed by cervical dislocation and the liver was dissected and immediately frozen in liquid nitrogen. The frozen liver was pulverized with dry ice to a fine powder with a blender and separated into tubes. Total RNAs were extracted from approximately 10 mg of liver using RNeasy Mini Kit (74106, QIAGEN) and QIAshredder (79656, QIAGEN). The amount and quality of RNAs were assessed using Nanodrop (Thermo Fisher Scientific) and the 2100 Bioanalyzer (Agilent Technologies). The cDNA libraries were prepared using the TruSeq Stranded mRNA Kit (Illumina, San Diego, CA, USA). The resulting cDNAs were subjected to 150 base pair (bp) paired-end sequencing on an Illumina NovaSeq6000 Platform (Illumina). Note that data at 0 hours are identical with PSUB019783.