Cut & Run-seq analysis for transcription factors overexpression in GI-MEN cells

Cut & Run analysis was performed in an neuroblastoma cell line to analyze DNA bindings of ASCL1-tag-HA in GI-MEN ASCL1-tag-HA cells and GI-MEN ASCL1-tag-HA+4TFs cells; analyze DNA bindings of MYCN, PHOX2B and H3K27ac in, GI-MEN 4TFs cells, and GI-MEN ASCL1-tag-HA+4TFs cells. CUT&RUN was performed using an EpiCypher kit (SKU: 14-1048). Approximately 0.5 million cells were used for each reaction. Antibody targeting the transcription factor or histone modification of interest as well as IgG were used. DNA was purified for library construction using an Illumina sequencing library kit. Illumina HiSeq sequencing of ~10 M paired-end 150-bp reads was performed at Genewiz.