Acidosis-induced gene expression regulation in human epithelial cells, fibroblasts and aortic vascular smooth muscle cells (in monoculture)

Acidic milieu induced gene expression regulation in HK2 cells (derived from the proximal tubule), CCD-1092SK (foreskin fibroblasts) and HAoSMC (primary human aortic smooth muscle cells) Overall design: HK2, CCD-1092SK and HAoSMC were cultivated with an acidic milieu (pH 6.4 or 6.8) for 48h, before proceeding with RNAseq. pH 7.4 served as reference for all cell types.