Shifts in the Immunoepigenomic Landscape of Monocytes in Response to a Diabetes-Specific Social Support Intervention: A Pilot Study Among Native Hawaiian Adults with Diabetes

Genome-wide DNA methylation profiling of bead-enriched total monocytes collected from Native Hawaiian participants with known type 2 diabetes mellitus enrolled in a 3 month diabetes-specific social support education intervention. DNA methylation profiling was performed across ~450,000 CpGs from monocytes using the Illumina Infinium HumanMethylation450 BeadChip. Samples included 8 participants with paired DNA methylation data collected at pre-intervention and post-intervention (3 months), and 2 non-diabetic donors. 500 ng of genomic DNA from enriched total monocytes were bisulfite converted (EZ DNA Methylation Kit - Zymo Research), bisulfite converted DNA was randomly assigned to a chip well on the HumanMethylation450 BeadChip, and imaged with the iScan SQ instrument (Illumina) to obtain raw image intensities.