Cis-SAGe fusion RNAs in transcription splicing factors knocking-down 293T cells

Total RNA extracted from 15 knocking down treated 293T cells using siRNAs targeting transcription splicing factors and sequenced using Illumina Hiseq PE150 platform to generate RNA sequencing with 150bp in read length. Nearly 50 million raw reads were yielded from each sample respectively. We used FastQC to confirm the quality of raw fastq sequencing data, and Ericscript software to detect fusion transcripts. Overall design: Analysis of Cis-SAGe fusion RNAs in the 293T cells with knocking down transcription splicing factors