DEGs are classified by gene families that influence (A) immune responses and (B) epithelial barrier formation and maintenance.

A-B. Cells were analyzed by RNA-sequencing and gene expression was compared between IBH-KER and H-KER stimulated with allergic cytokine milieu (ACM) or unstimulated keratinocytes derived from IBH-affected and H-horses. In (A) representative genes of immune responses and (B) epithelial barrier genes are shown. (Pink = statistically significant upregulation and log2 fold change >1; beige = statistically significant upregulation and log2 fold change <1; dark blue = statistically significant downregulation and log2 fold change >-1; light blue = statistically significant downregulation and log2 fold change <-1; gray = no difference in gene expression; false discovery rate <0.05). Log2 fold changes are noted for all DEGs.