Single-cell RNA-seq reveals the keratinocyte heterogeneity and increased interfollicular basal keratinocytes in wound-induced hair follicle neogenesis

We created large wounds (LW) and small wounds (SW) on the dorsal side of mice respectively, and hair follicle regeneration was confirmed by whole mount assay and HE staining. The re-epithelized tissues of 3 LW mice and 3 SW mice were collected for single-cell RNA sequencing analysis. Results were corroborated by immunofluorescence (IF) staining and masson staining.本研究分别在小鼠背部构建了大面积创伤（large wounds, LW）与小面积创伤（small wounds, SW），并通过全铺片染色实验（whole mount assay）与苏木精-伊红染色（HE staining）验证了毛囊再生情况。随后收集3只LW组小鼠与3只SW组小鼠的再上皮化组织，开展单细胞RNA测序分析。本研究结果通过免疫荧光（IF）染色与马松三色染色（Masson staining）得到了佐证。