Costa et al. Multiple mating rescues offspring sex ratio but not productivity in a haplodiploid exposed to developmental heat stress

Summary:
The data and scripts presented were used to assess how a sublethal high temperature (36°C) affects the reproductive system of a haplodiploid species. Using spider mites (Tetranychus urticae) developed either at 25°C or 36°C, we paired individuals in four treatments (♀25°C x ♂25°C, ♀25°C x ♂36°C, ♀36°C x ♂25°C, ♀36°C x ♂36°C), to measure the effect of temperature on the fertility of both sexes, mating and remating eagerness, and the paternity of the offspring of females with different mating histories.

Files available:
We provide 3 .R files (Script_statistical_analysis_Fertility_and_Paternity_Share, Script_statistical_analysis_Mating_Behavior, Script_figures) and 6 .csv files (Dataset_Fertility_and_Paternity_share, Dataset_altered_Fertility_and_Paternity_share, Dataset_means_Fertility_and_Paternity_share, Dataset_Mating_behavior, Dataset_means1_Mating_behavior, Dataset_means2_Mating_behavior).

Script_statistical_analysis_Fertility_and_Paternity_Share
This file contains the script used to make the statistical analysis for the Fertility and Paternity Share experiment.

Script_statistical_analysis_Mating_Behavior
This file contains the script used to make the statistical analysis for the Mating Behavior experiment.

Script_figures
This file contains the script used to create all the figures.

Dataset_Fertility_and_Paternity_share
This file contains the raw data of the effect of temperature on fertility and paternity share.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated).
Rep - replicate number per treatment.
Block - day in which matings were observed.
Rtemp - temperature of the observation room.
Rhum - humidity of the observation room.
Fecundity - number of eggs laid during 4 days per female.
Unhatched_eggs - number of eggs that did not develop.
Total_daughters - number of adult daughters counted.
Total_sons - number of adult sons counted.
Total_contribution_males - sum of adult daughters and unhatched eggs.
Total_offspring - sum of adult daughters, adult sons and unhatched eggs.
Prop_daughters_ue - proportion of adult daughters in the fertilized offspring (Total_daughters / Total_contribution_males).
Prop_fert_off - proportion of adult daughters and unhatched eggs in the offspring (Total_contribution_males / Total_offspring).
Observations - any observations made in each replicate.

Dataset_altered_Fertility_and_Paternity_share
This file contains the same raw data as Dataset_Fertility_and_Paternity_share, and includes 4 more lines (in SM from each treatment) to deal with the lack of variance in Total_daughters and Unhatched_eggs, which impeded the statistical analysis.
Table headers are similar to the ones in Dataset_Fertility_and_Paternity_share.

Dataset_means_Fertility_and_Paternity_share
This file contains the means of each variable studied for the effect of temperature on fertility and paternity share.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the first male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated).
Mean_prop_fem_ue - mean proportion of daughters and unhatched eggs in the offspring per treatment and female mating history.
Mean_total_off - mean number of offspring (daughters, sons and unhatched eggs) per treatment and female mating history.
Mean_prop_fem - mean proportion of adult daughters in the fertilized offspring (daughters and unhatched eggs) per treatment and female mating history.

Dataset_Mating_behavior
This file contains the raw data of the effect of temperature on mating behavior.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; MM, if multiple mated).
Rep - replicate number per treatment.
Block - day in which matings were observed.
Rtemp - temperature of the observation room.
Rhum - humidity of the observation room.
Mating_rate - number of times the female mated.
Lat_sec1 - latency to the first copulation of the female.
CopYN - if the female mated a first time (1=yes, 0=no).
Cop_sec1 - duration of the first copulation of the female.
Lat_sec2 - latency to the second copulation of the female.
Cop2YN - if the female mated a second time (1=yes, 0=no).
Cop_sec2 - duration of the second copulation of the female.
Fecundity - number of eggs laid during 4 days per female.
Total_daughters - number of adult daughters counted.
Total_sons - number of adult sons counted.
Sex_ratio - proportion of adult daughters in the offspring (adult daughters and sons).
Total_adults - sum of adult daughters and sons.
Observations - any observations made in each replicate.

Dataset_means1_Mating_behavior
This file contains the means of each variable studied for the effect of temperature on mating behavior.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the first male (25°C or 36°C).
Mean_lat1 - mean latency to the first copulation of the female per treatment.
Mean_cop1 - mean duration of the first copulation of the female per treatment.
Mean_lat2 - mean latency to the second copulation of the female per treatment.
Mean_cop2 - mean duration of the second copulation of the female per treatment.

Dataset_means2_Mating_behavior
This file contains the means of each variable studied for the effect of temperature on mating behavior present in the supplementary material.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the first male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; MM, if multiple mated).
Mean_off - mean number of offspring (adult daughters and sons) per treatment and female mating history.
Mean_sexratio - mean proportion of adult daughters in the offspring (adult daughters and sons) per treatment and female mating history.

摘要：本数据集及脚本用于评估亚致死高温（36°C）对单倍二倍体物种生殖系统的影响。本研究采用在25°C或36°C下发育的蜘蛛螨（Tetranychus urticae），将个体分为四组处理（♀25°C x ♂25°C、♀25°C x ♂36°C、♀36°C x ♂25°C、♀36°C x ♂36°C），以测量温度对雌雄两性生育力、交配及再交配意愿，以及不同交配历史雌性后代父系贡献的影响。

文件清单：

我们提供了3个.R文件（Script_statistical_analysis_Fertility_and_Paternity_Share、Script_statistical_analysis_Mating_Behavior、Script_figures）和6个.csv文件（Dataset_Fertility_and_Paternity_share、Dataset_altered_Fertility_and_Paternity_share、Dataset_means_Fertility_and_Paternity_share、Dataset_Mating_behavior、Dataset_means1_Mating_behavior、Dataset_means2_Mating_behavior）。

Script_statistical_analysis_Fertility_and_Paternity_Share

此文件包含用于进行生育力和父系贡献份额实验的统计分析的脚本。

Script_statistical_analysis_Mating_Behavior

此文件包含用于进行交配行为实验的统计分析的脚本。

Script_figures

此文件包含创建所有图表的脚本。

Dataset_Fertility_and_Paternity_share

此文件包含温度对生育力和父系贡献份额影响的原始数据。

表头解释如下：

Temp_fxm_1M - 雌性和雄性配对的发育温度（雌性首次交配）。
Temp_fem - 雌性的发育温度（25°C或36°C）。
Temp_male - 雄性的发育温度（25°C或36°C）。
Type_M - 雌性的交配历史（SM，若单次交配；DM，若两次交配；MM，若多次交配）。
Rep - 每个处理的重复次数。
Block - 观察交配的日期。
Rtemp - 观察室的温度。
Rhum - 观察室的湿度。
Fecundity - 每只雌性在4天内产卵的数量。
Unhatched_eggs - 未发育的卵的数量。
Total_daughters - 计数的成年女儿数量。
Total_sons - 计数的成年儿子数量。
Total_contribution_males - 成年女儿和未孵化卵的总和。
Total_offspring - 成年女儿、成年儿子和未孵化卵的总和。
Prop_daughters_ue - 成年女儿在受精后代中的比例（Total_daughters / Total_contribution_males）。
Prop_fert_off - 成年女儿和未孵化卵在后代中的比例（Total_contribution_males / Total_offspring）。
Observations - 每个重复中的观察结果。

Dataset_altered_Fertility_and_Paternity_share

此文件包含与Dataset_Fertility_and_Paternity_share相同的原始数据，并增加了4行（每个处理的SM），以解决Total_daughters和Unhatched_eggs方差不足的问题，这阻碍了统计分析。

表头与Dataset_Fertility_and_Paternity_share中的类似。

Dataset_means_Fertility_and_Paternity_share

此文件包含研究温度对生育力和父系贡献份额影响的每个变量的平均值。

表头解释如下：

Temp_fxm_1M - 雌性和雄性配对的发育温度（雌性首次交配）。
Temp_fem - 雌性的发育温度（25°C或36°C）。
Temp_male - 首次雄性的发育温度（25°C或36°C）。
Type_M - 雌性的交配历史（SM，若单次交配；DM，若两次交配；MM，若多次交配）。
Mean_prop_fem_ue - 每个处理和雌性交配历史中后代中女儿和未孵化卵的平均比例。
Mean_total_off - 每个处理和雌性交配历史中后代的平均数量。
Mean_prop_fem - 每个处理和雌性交配历史中受精后代中成年女儿的平均比例。

Dataset_Mating_behavior

此文件包含温度对交配行为影响的原始数据。

表头解释如下：

Temp_fxm_1M - 雌性和雄性配对的发育温度（雌性首次交配）。
Temp_fem - 雌性的发育温度（25°C或36°C）。
Temp_male - 雄性的发育温度（25°C或36°C）。
Type_M - 雌性的交配历史（SM，若单次交配；MM，若多次交配）。
Rep - 每个处理的重复次数。
Block - 观察交配的日期。
Rtemp - 观察室的温度。
Rhum - 观察室的湿度。
Mating_rate - 雌性交配的次数。
Lat_sec1 - 雌性首次交配的潜伏期。
CopYN - 雌性是否首次交配（1=是，0=否）。
Cop_sec1 - 雌性首次交配的持续时间。
Lat_sec2 - 雌性第二次交配的潜伏期。
Cop2YN - 雌性是否第二次交配（1=是，0=否）。
Cop_sec2 - 雌性第二次交配的持续时间。
Fecundity - 每只雌性在4天内产卵的数量。
Total_daughters - 计数的成年女儿数量。
Total_sons - 计数的成年儿子数量。
Sex_ratio - 后代中成年女儿的比例（成年女儿和儿子）。
Total_adults - 成年女儿和儿子的总和。
Observations - 每个重复中的观察结果。

Dataset_means1_Mating_behavior

此文件包含研究温度对交配行为影响的每个变量的平均值。

表头解释如下：

Temp_fxm_1M - 雌性和雄性配对的发育温度（雌性首次交配）。
Temp_fem - 雌性的发育温度（25°C或36°C）。
Temp_male - 首次雄性的发育温度（25°C或36°C）。
Mean_lat1 - 每个处理中雌性首次交配的平均潜伏期。
Mean_cop1 - 每个处理中雌性首次交配的平均持续时间。
Mean_lat2 - 每个处理中雌性第二次交配的平均潜伏期。
Mean_cop2 - 每个处理中雌性第二次交配的平均持续时间。

Dataset_means2_Mating_behavior

此文件包含补充材料中温度对交配行为影响的每个变量的平均值。

表头解释如下：

Temp_fxm_1M - 雌性和雄性配对的发育温度（雌性首次交配）。
Temp_fem - 雌性的发育温度（25°C或36°C）。
Temp_male - 首次雄性的发育温度（25°C或36°C）。
Type_M - 雌性的交配历史（SM，若单次交配；MM，若多次交配）。
Mean_off - 每个处理和雌性交配历史中后代的平均数量。
Mean_sexratio - 每个处理和雌性交配历史中后代中成年女儿的比例（成年女儿和儿子）。