Gene expression data of macrophages cultured on porous titanium discs

Titanium is a common implant material. However, in some patients titanium implants fail. Macrophages are key cells involved in foreign body response. To identify macrophage response to titainum, primary human macrophages were cultured on porous titanium discs for 6 days We used microarrays to determine the global expression pattern induced by porous titanium in macrophages and identify potential genes involved in implant failure. PBMC derived monocytes were cultured on cell culture plastic or on porous titanium discs for 6 days. Cells were stimulated either with IFNgamma or IL-4 or left unstimulated. On the 6th day RNA was isolated and whole transcriptome was analyzed using Affymetrix microarrays