RT-qPCR verification of the microarray data set.

Genes expressed in the cornea were examined for gene expression by quantitative polymerase chain reaction following reverse transcription of total RNA (RT-qPCR) prepared from the adult zebrafish cornea and the dermis. Expression was normalized by using the level of expression of the ef1a gene as an internal control. The result is shown as the fold-change value of the expression level in the cornea in relation to the dermis. All genes presented in the table showed a direction of enrichment consistent with the microarray results (three biological repeats, two tail unpaired t-test, p-adjusted <0.05).
a) The label “unique” in the fold-change value indicates the absence of detectable transcripts in the dermis and hence exclusive expression in the cornea (rpe65a, otx1a, col9a2 and epcam).
RT-qPCR verification of the microarray data set.