Expression data from the mouse liver with constitutively active FoxO3

Activation of hepatic FoxO3 signaling induces hyperglycemia and hyperinsulinemia. Metformin administration reverses FoxO3-induced phenotype development. To investigate the underlying mechanism, we used microarrays to detail the global programme of gene expression which is regulated by hepatic FoxO3 signaling. We generated transgenic mice which express a constitutively active FoxO3 controlled under the LAP promotor. The liver samples were collected from transgenic mice as well as from control animals at the age of 7 weeks. RNA extraction was performed from these liver samples and hybridized on Affymetrix microarrays.