PacBio_sequencing_of_M__tuberculosis_

Whole genome sequencing of Mycobacterium tuberculosis is being used in public health to identify tuberculosis transmission clusters and in clinical microbiology to reveal the presence of molecular determinants of drug resistance. In both cases, Illumina sequencing is the most common platform for sequencing due to the high clonality of the Mycobacterium tuberculosis genomes. Long-read, single-molecule technologies have the potential to overcome some limitations of Illumina short reads but at the same time need to show that they can match some of the major advantages of Illumina sequencing. We want to investigate the potential role of long-read sequencing on public health and clinical microbiology of tuberculosis.