Gene expression in glioblastoma cell with sh-lncEPAT

Depleting lncEPAT in GBM cells leads to altered gene expression profile lncRNAs are aberrantly expressed in different cancers; recent discoveries showed that lncRNAs participate in cancer signaling pathways via interacting with proteins, nucleotides, and lipids. Our goal is to characterize the role of an oncogenic lncRNA (lncEPAT) that mediates the integration of the dysregulated EGFR pathway with H2A deubiquitination in glioblastoma tumorigenesis. GSC23 cells were transduced with sh-Control and sh-lncEPAT lentivirus and selected with puromycin. The RNAs of tumor cells were obtained and Affymetrix microarrays was performed to explore consequent gene expression pattern change.