Single-Cell Atlas of the Tumor Immune Microenvironment Across Syngeneic Murine Models

The tumor immune microenvironment plays a critical role in tumor progression and responses to immunotherapy. Nevertheless, its cellular complexity and heterogeneity remain incompletely understood. In this study, we employed high-resolution single-cell RNA sequencing on CD45+ immune cells isolated from ten syngeneic murine tumor models, representing seven distinct cancer types under treatment-naïve conditions, thereby enabling a comprehensive profiling of tumor-infiltrating immune cells. We classified seven principal immune cell populations and provided an in-depth characterization of T cells, NK/innate lymphoid cells, dendritic cells, monocytes/macrophages, and neutrophils. Overall design: Single-cell RNA sequencing (scRNA-seq) was performed on CD45+ immune cells isolated from ten treatment-naïve murine models. These models encompassed seven prevalent cancer types: breast mammary carcinoma (4T1, EMT6, MMTV-PyMT), colon carcinoma (CT26.WT, MC38), glioma (GL261), renal adenocarcinoma (Renca), lung carcinoma (LL2), melanoma (B16F10), and pancreatic adenocarcinoma (Pan02).